Linkam Scientific Instruments Limited | Automated tools for the freezing and screening of your cryo-samples with CryoGenium and CMS196V4

Booth 1542
In this workshop we will showcase two novel systems – our automated CryoGenium vitrification robot for freezing samples on EM grids as well as a new cryo-stage for cryo-fluorescence which we will show in the US for the first time.

The preparation of cryo-samples for Single Particle Analysis (SPA), cryo-EM tomography and cryo-fluorescence can be a challenge. The established sample freezing techniques based on blotting often suffer from inconsistent results and long optimisation cycles especially for new samples. To address this, we developed CryoGenium, an automated machine to perform grid handling, glow discharge and plunging based on a blot-free principle. Instead of blotting paper CryoGenium uses miniature suction nozzles to adjust the film thickness of the sample, enabling a lower amount of sample per grid as well as real-time control of the process and prediction of sample quality from the integrated optical microscope before taking the sample to the EM. CryoGenium can be used for SPA and also cell-based workflows. (See also https://www.linkam.co.uk/cryogenium.)

The cryo-stage CMS196V4 is the successor of our previous CLEM system for cryo-fluorescence with more than 150 installations world-wide. We will show the stage and highlight the multiple new features and improvements including better drift performance, improved handling, novel user interface concept and a new LN2 dispensing system.