Cryo Electron Microcopy (Cryo-EM) is an advanced imaging technique used to determine the three-dimensional structure of biomolecules at near atomic resolution. Quality of samples and the sample vitrification process are two factors that can significantly influence the results obtained by cryo-EM. Negative stain imaging is an invaluable part of the workflow, allowing researchers to easily triage samples at room temperature before going into cryo-EM. The Phenom desktop STEM offers a simple plug-and-play solution for screening negative stain samples in preparation for cryo-EM work. Part of this presentation will focus on how Phenom desktop STEM could replace the traditional TEM infrastructure and speed up negative stain screening workflow while simultaneously lowering cost.
VitroJet has evolved into a powerful efficiency tool for cryo-EM scientists to vitrify their samples. With real-time feedback and visualization of the grid and sample deposition process, users can analyze and tune their ice thickness on the fly. The second half of the presentation will focus on how VitroJet can transform the sample vitrification process, getting better Cryo-EM data in a faster more controllable way.
